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pcmv flex inverted teton3g 3xha flex inverted  (Addgene inc)


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    Addgene inc pcmv flex inverted teton3g 3xha flex inverted
    Pcmv Flex Inverted Teton3g 3xha Flex Inverted, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/teton3g/pCAG-Cre-IRES2-GFP+(Plasmid+%2326646)/us12570703-295-19-22
    Average 93 stars, based on 76 article reviews
    pcmv flex inverted teton3g 3xha flex inverted - by Bioz Stars, 2026-09
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    Related Articles

    Transfection:

    Article Title: On demand expression control of endogenous genes with DExCon, DExogron and LUXon reveals differential dynamics of Rab11 family members
    Article Snippet: Recombinant DNA reagent , pCDH-TRE3GS-EF1a-tagBFP-T2A-TetOn3G , Andrew Gilmore lab , , Lentiviral tetracycline inducible backbone. .. Transfected construct (synthetic) , pCDH-EF1a-tagBFP-T2A-TetOn3G , this paper , RRID: Addgene_179888 , Lentiviral construct to express TetOn3G. .. Transfected construct (synthetic) , pCDH-EF1a-HygroR-T2A-TetOn3G , this paper , RRID: Addgene_179887 , Lentiviral construct to express TetOn3G.

    Article Title: On demand expression control of endogenous genes with DExCon, DExogron and LUXon reveals differential dynamics of Rab11 family members
    Article Snippet: Transfected construct (synthetic) , pCDH-EF1a-tagBFP-T2A-TetOn3G , this paper , RRID: Addgene_179888 , Lentiviral construct to express TetOn3G. .. Transfected construct (synthetic) , pCDH-EF1a-HygroR-T2A-TetOn3G , this paper , RRID: Addgene_179887 , Lentiviral construct to express TetOn3G. .. Transfected construct (human) , mCherry-Rab11a , Michael Davidson lab , RRID: Addgene_55124 , Mammalian Expression vector for transfection.

    Construct:

    Article Title: On demand expression control of endogenous genes with DExCon, DExogron and LUXon reveals differential dynamics of Rab11 family members
    Article Snippet: Recombinant DNA reagent , pCDH-TRE3GS-EF1a-tagBFP-T2A-TetOn3G , Andrew Gilmore lab , , Lentiviral tetracycline inducible backbone. .. Transfected construct (synthetic) , pCDH-EF1a-tagBFP-T2A-TetOn3G , this paper , RRID: Addgene_179888 , Lentiviral construct to express TetOn3G. .. Transfected construct (synthetic) , pCDH-EF1a-HygroR-T2A-TetOn3G , this paper , RRID: Addgene_179887 , Lentiviral construct to express TetOn3G.

    Article Title: On demand expression control of endogenous genes with DExCon, DExogron and LUXon reveals differential dynamics of Rab11 family members
    Article Snippet: Transfected construct (synthetic) , pCDH-EF1a-tagBFP-T2A-TetOn3G , this paper , RRID: Addgene_179888 , Lentiviral construct to express TetOn3G. .. Transfected construct (synthetic) , pCDH-EF1a-HygroR-T2A-TetOn3G , this paper , RRID: Addgene_179887 , Lentiviral construct to express TetOn3G. .. Transfected construct (human) , mCherry-Rab11a , Michael Davidson lab , RRID: Addgene_55124 , Mammalian Expression vector for transfection.

    Synthesized:

    Article Title: Engineered Feedback Employing Natural Hypoxia-Responsive Factors Enhances Synthetic Hypoxia Biosensors.
    Article Snippet: .. A previously characterized HBS20 was synthesized as overlapping oligonucleotides, as were the minimal promoters YB_TATA and CMV_min.31 The minimal promoter SV40_min, the EF1α and TRE3GV promoters, the genes encoding the stable HIF1α mutant, TetON3G, BlastR, EBFP2, and EYFP; and the CHS4 insulator were sourced from plasmids from Addgene and Clontech as described in Supporting Table 11.31,42,53−56 The genes for mTagBFP2,57 mNeonGreen,58 mRuby3,59 miRFP670,60,61 and miRFP72062 were synthesized as codon-optimized Gene Strings by Thermo Fisher. .. A previously characterized HBS20 was synthesized as overlapping oligonucleotides, as were the minimal promoters YB_TATA and CMV_min.31 The minimal promoter SV40_min, the EF1α and TRE3GV promoters, the genes encoding the stable HIF1α mutant, TetON3G, BlastR, EBFP2, and EYFP; and the CHS4 insulator were sourced from plasmids from Addgene and Clontech as described in Supporting Table 11.31,42,53−56 The genes for mTagBFP2,57 mNeonGreen,58 mRuby3,59 miRFP670,60,61 and miRFP72062 were synthesized as codon-optimized Gene Strings by Thermo Fisher.

    Mutagenesis:

    Article Title: Engineered Feedback Employing Natural Hypoxia-Responsive Factors Enhances Synthetic Hypoxia Biosensors.
    Article Snippet: .. A previously characterized HBS20 was synthesized as overlapping oligonucleotides, as were the minimal promoters YB_TATA and CMV_min.31 The minimal promoter SV40_min, the EF1α and TRE3GV promoters, the genes encoding the stable HIF1α mutant, TetON3G, BlastR, EBFP2, and EYFP; and the CHS4 insulator were sourced from plasmids from Addgene and Clontech as described in Supporting Table 11.31,42,53−56 The genes for mTagBFP2,57 mNeonGreen,58 mRuby3,59 miRFP670,60,61 and miRFP72062 were synthesized as codon-optimized Gene Strings by Thermo Fisher. .. A previously characterized HBS20 was synthesized as overlapping oligonucleotides, as were the minimal promoters YB_TATA and CMV_min.31 The minimal promoter SV40_min, the EF1α and TRE3GV promoters, the genes encoding the stable HIF1α mutant, TetON3G, BlastR, EBFP2, and EYFP; and the CHS4 insulator were sourced from plasmids from Addgene and Clontech as described in Supporting Table 11.31,42,53−56 The genes for mTagBFP2,57 mNeonGreen,58 mRuby3,59 miRFP670,60,61 and miRFP72062 were synthesized as codon-optimized Gene Strings by Thermo Fisher.

    Plasmid Preparation:

    Article Title: SYNGAP1 deficiency disrupts synaptic neoteny in xenotransplanted human cortical neurons in vivo
    Article Snippet: Human H9 embryonic stem cells (WiCell) were routinely propagated on mitotically inactivated mouse embryonic fibroblasts in Knockout DMEM (Thermo Fisher Scientific) supplemented with 20% Knockout Serum Replacement (Thermo Fisher Scientific), 1X Non-essential Amino Acids (Thermo Fisher Scientific), 1X Penicillin/Streptomycin (Thermo Fisher Scientific), 1X 2-Mercaptoethanol (Merck), 2mM L-glutamine (Thermo Fisher Scientific). .. pLenti-TRE3G-GCaMP7b-WPRE: The TRE3G fragment (pLenti CMVTRE3G eGFP Puro (w819-1) was a gift from Eric Campeau (Addgene plasmid # 27570; http://n2t.net/addgene:27570 ; RRID:Addgene_27570)) and the GCaMP7b fragment (pGP-AAV- syn -jGCaMP7b-WPRE was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 104489; http://n2t.net/addgene:104489 ; RRID:Addgene_104489)) was transferred to lentiviral backbone by restriction enzyme digestion and ligation. pLenti-hSynI-TetON3G-WPRE: The DNA fragment of TetON3G was amplified from AAVS1-TRE3G-EGFP was a gift from Su-Chun Zhang (Addgene plasmid # 52343; http://n2t.net/addgene:52343 ; RRID:Addgene_52343) using following primers pair: 5′-GTCGACatgtctagactggacaagag-3’/5′-ACGCGTttacccggggagcatgtcaa-3’. ..

    Ligation:

    Article Title: SYNGAP1 deficiency disrupts synaptic neoteny in xenotransplanted human cortical neurons in vivo
    Article Snippet: Human H9 embryonic stem cells (WiCell) were routinely propagated on mitotically inactivated mouse embryonic fibroblasts in Knockout DMEM (Thermo Fisher Scientific) supplemented with 20% Knockout Serum Replacement (Thermo Fisher Scientific), 1X Non-essential Amino Acids (Thermo Fisher Scientific), 1X Penicillin/Streptomycin (Thermo Fisher Scientific), 1X 2-Mercaptoethanol (Merck), 2mM L-glutamine (Thermo Fisher Scientific). .. pLenti-TRE3G-GCaMP7b-WPRE: The TRE3G fragment (pLenti CMVTRE3G eGFP Puro (w819-1) was a gift from Eric Campeau (Addgene plasmid # 27570; http://n2t.net/addgene:27570 ; RRID:Addgene_27570)) and the GCaMP7b fragment (pGP-AAV- syn -jGCaMP7b-WPRE was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 104489; http://n2t.net/addgene:104489 ; RRID:Addgene_104489)) was transferred to lentiviral backbone by restriction enzyme digestion and ligation. pLenti-hSynI-TetON3G-WPRE: The DNA fragment of TetON3G was amplified from AAVS1-TRE3G-EGFP was a gift from Su-Chun Zhang (Addgene plasmid # 52343; http://n2t.net/addgene:52343 ; RRID:Addgene_52343) using following primers pair: 5′-GTCGACatgtctagactggacaagag-3’/5′-ACGCGTttacccggggagcatgtcaa-3’. ..

    Amplification:

    Article Title: SYNGAP1 deficiency disrupts synaptic neoteny in xenotransplanted human cortical neurons in vivo
    Article Snippet: Human H9 embryonic stem cells (WiCell) were routinely propagated on mitotically inactivated mouse embryonic fibroblasts in Knockout DMEM (Thermo Fisher Scientific) supplemented with 20% Knockout Serum Replacement (Thermo Fisher Scientific), 1X Non-essential Amino Acids (Thermo Fisher Scientific), 1X Penicillin/Streptomycin (Thermo Fisher Scientific), 1X 2-Mercaptoethanol (Merck), 2mM L-glutamine (Thermo Fisher Scientific). .. pLenti-TRE3G-GCaMP7b-WPRE: The TRE3G fragment (pLenti CMVTRE3G eGFP Puro (w819-1) was a gift from Eric Campeau (Addgene plasmid # 27570; http://n2t.net/addgene:27570 ; RRID:Addgene_27570)) and the GCaMP7b fragment (pGP-AAV- syn -jGCaMP7b-WPRE was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 104489; http://n2t.net/addgene:104489 ; RRID:Addgene_104489)) was transferred to lentiviral backbone by restriction enzyme digestion and ligation. pLenti-hSynI-TetON3G-WPRE: The DNA fragment of TetON3G was amplified from AAVS1-TRE3G-EGFP was a gift from Su-Chun Zhang (Addgene plasmid # 52343; http://n2t.net/addgene:52343 ; RRID:Addgene_52343) using following primers pair: 5′-GTCGACatgtctagactggacaagag-3’/5′-ACGCGTttacccggggagcatgtcaa-3’. ..



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